EndomeTRIO

What is the endometrium?

The endometrium is the mucosal layer of the uterus in which the fertilised egg implants. This means that the endometrium, also called the uterine mucosa, found in the innermost portion of the uterine wall, is a key factor for a successful pregnancy. The uterine mucosa is typically evaluated using ultrasound, but ultrasound can only determine its height and structure, however bacterial flora (the microbiome) and the so-called implantation window – i.e. the best possible time to transfer an embryo into the uterus – are also important.

A hormonal imbalance in a woman’s body (the dominance of oestrogen over progesterone) can cause the uterine mucosa to grow outside of the uterus. This condition is called endometriosis.

What is EndomeTRIO?

EndomeTRIO is an examination that includes three tests from one sample of the uterine mucosa.

ALICE (Analysis of Infectious Chronic Endometritis)

The balance of bacteria in the endometrium is important for successful implantation of an embryo. If pathogenic bacteria are present in the uterus, this balance can be disrupted.

The ALICE test can reveal the presence of bacteria that can be causing a chronic infection. These infections, present in up to 30% of infertile women, can be one of the causes for recurring miscarriages. A proper treatment plan, however, can resolve this situation.

EMMA (Endometrial Microbiome Metagenomic Analysis)

Taking a sample of the uterine mucosa and its subsequent analysis using next generation sequencing (NGS) can reveal other bacteria that can also cause infertility in women.

The EMMA test provides a comprehensive overview of the makeup of the uterine mucosa. In the event of less-than-optimal representation of bacteria, treatment using antibiotics or probiotics may be recommended.

ERA (Endometrial Receptivity Analysis)

Recurring unsuccessful embryonic transfers can be difficult for a woman, both physically and psychologically. Despite the fact that the treated couple has undergone numerous tests and their embryos are ready for transfer to the uterus according to the results of pre-implantation genetic testing, the uterine mucosa may not be ready for the transfer.

The ERA test determines the optimal window of time when the uterine mucosa is receptive to accepting an embryo. In other words, it establishes the optimal time for the embryo transfer. Its results will also help establish suitable hormonal preparation and its duration. This test helps achieve a 73% success rate (Implantation Rate) in patients with recurring implantation failure.